Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1. Antibacterial and antifungal activities of crude extracts were determined by disc diffusion method. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens. Rebeccas data presence of a halo shows that the oil inhibits the growth of the bacteria. The results obtained from the present study provide evidence that ethanolic extracts of s. Indian plant extracts antimicrobial properties superior to. Methanol, ethylacetate extracts of plant species heracleum crenatifolium, h.
L from each plant crude extract 500 mg ml1 was added into each well on the seeded medium and allowed to stand on the bench for 1 h for proper diffusion and thereafter incubated at 37 o c for 24 h. The evolution and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, is of great concern to the global health community. In vitro antibacterial activity of crude extracts from. Antimicrobial activities of the oyster mushroom extracts against bacillus cereus, streptococcus agalactiae, agrobacterium, pseudomonas aeruginosa, escherichia coli and shigella dysenteriae were. For more detail information please read the manuscript. A study on phytochemical analysis and antimicrobial activity of hyptis suaveolens l. Garlic extract, when combined with ampicillin, revealed some effects on klebsiella pneumoniae whereas proteus sp. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science in the subject life sciences at the university of south africa supervisor. Screening of antibacterial activity of the medicinal plant. Two grams of this crude extract were reduced to powder using a porcelain mortar, suspendeddissolved in 10 ml distilled water and filtered through a paper filter whatman, 80 gm2, porosity 3 m. Plant extracts have shown inhibitory effect on the growth of the bacteria studied, although of distinct forms. Antibacterial activity of petalidium oblongifolium and the isolation of a bioactive compound 25 3. Antimicrobial and antioxidant activity of leaf extracts of.
Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. Effect of fractioning on antibacterial activity of enantia chlorantha. Ethanol extract six grams of the powdered plant material was extracted in a soxhlet extractor using 25ml of 40% ethanol. Antibacterial and phytochemical studies on twelve species of indian. Boiled water extracts of guava leaf showed the largest zone of inhibition 22 mm against v. The extract of 50 g of dried plant materials with ethanol yielded plant extract residues ranged from 1. The synergistic effect between plant extracts clove, jambul syzygium cumini, pomegranate and thyme and some antimicrobial drugs was also evaluated. The extract was filtered using what man filter paper no.
Antimicrobial activity of fermented theobroma cacao pod husk. Antibacterial activity of leaf extracts of baeckea. The highest antibacterial potentiality was exhibited by the methanolic leaf extract of t. The assay for antibacterial activity of each plant leaf extract was tested by disc diffusion method kirby et al. In addition to these assays, antifungal phytochemicals can be analyzed by a spore germination assay. It has antiinflammatory, antitumor, antispasmodic properties, and acts as an antioxidant. From the results of the disc diffusion screening, b. Antimicrobial activity of medicinal plant leaf extracts. Acetone technical grade, merck was used as an extractant in the assays using a ratio of 1. The aim of the study is to select an active plant extract. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a.
Alternative remediation could include new antimicrobial substances that are effective, available, and low in toxicity. Medicinal plants, hydromethanolic extracts, antibacterial. The remaining nondissolved material was resubmitted to the same pro cedure twice to yield a total of 30 ml waterdissolved extract. Water extracts of green tea leaf at boiling and room temperature showed 17. The highest antibacterial activity was exhibited by methanol cold extract against staphylococcus epidermidis 15 mm and bacillus cereus 14 mm respectively. This study explores the antibacterial properties of plants used. Hot hexane extract showed an inhibition zone of 12 mm against. Antibacterial activity of some medicinal plant extracts. This work was therefore carried out to determine the antibacterial effect of d. Antibacterial properties, secondary metabolites, multi drug resistant pathogens. Exactly one milliliter of each of the standardized test organism was transferred into different sterile petri dishes.
Efficacy of natural plant extracts in antimicrobial. Studies on some plant extracts for their antimicrobial. The plants screened were ocimum sanctum, jatropha gossypifolia, boerhavia diffusa, azadirachta indica, solidago virgaurea, and commelina benghalensis. Antibacterial activities of plant extracts were compared with those of standard antibiotics. Antibacterial characheristics of plants from heracleum genus have relatively slightly been researched. In vitro antibacterial activity of the crude methanol extract of.
Antimicrobial activity of wild olive crude extracts in vitro. Antibacterial activity of traditional medicinal plants. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Abdelshafy s 2014 evaluation of antibacterial effect of some sinai medicinal plant extracts on bacteria isolated from bovine mastitis, veterinary world, 711. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. It is therefore recommended that the nature and the number of the active antibacterial principles involved in each plant extract be studied in detail.
Phytochemical content, antibacterial and antioxidant potential of. Biochemistry graduates to have results from their final year. Among natural substances widespread in fruits, vegetables, spices, and medicinal plants, flavonoids and organic acids belong to the. Antibacterial activity of plant extracts and phytochemicals on. Plant extracts for the control of bacterial growth. Boiled water extract of neem leaf showed moderate zone of inhibition against escherichia coli 10 mm and. The resulting extracts were subsequently weighted to produce 0. Delonix elata, enicostemma axillare, merremia tridentata, mollugo cerviana and solanum incanum used in indian herbal medicine. Oregano essential oil, given in combination with according to world health organization who more than 80% of. European journal of botany plant sciences and phytology. All plant extracts showed no antibacterial activity with their high mic values against esblproducing bacteria and cre. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. The growth inhibition zone of the ethanol extract of rosemary was 24 to 27 mm, whereas the volatile oil of rosemary had no effect on a. The decrease in cytopathic effect in the presence of the plant extract is then measured.
Grapeseed sage lemon balm neem pine grapeseed grapeseed grapeseed tea tree emmas data aiyshas data. For evaluating antibacterial activity, both aqueous and organic solvent methanol was used. Mean zones of inhibition of different concentrations of neem leaf extract against s. Concentrations of neem leaf extract against mssa figure 2. Antimicrobial activity of some plant extracts against. In general, the antibiotic controls produced greater zones of inhibition except for povidone iodine 10% which antibacterial activity was comparable to the 100% extract on s. Concerns regarding the safety and toxicity of medicinal. Antibacterial activity of plant extracts and phytochemicals. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Most are secondary metabolites, of which at least 12,000 have been isolated, a number estimated to be less than 10% of the total. The plates were incubated uninvertedly at 370c for 24 hours. The results obtained with pseudomonas aeruginosa was particularly interesting, since it was inhibited by clove, jambolan, pomegranate and thyme extracts.
Antibacterial properties of alkaloid extracts from. Antibacterial activities of some plant extracts utilized in. Antibacterial effect of dodonea viscosa hop bush leaf. Antibacterial activities of some borneo plant extracts. Chemical composition and antibacterial activity of essential. A study on phytochemical analysis and antimicrobial. The extract exhibited antibacterial activity against the bacteria escherichia coli, bacillus subtilis, staphylococcus aureus and pseudomonas aeruginosa. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig.
Evaluation of antibacterial activity of some medicinal plants extracts. The highest yield of plant extract was obtained from punica granatum 4. A 25mgml of the plant extract was prepared and introduced into different holes bored on the sterile medium containing the test organism. Antibacterial activity of leaf extracts of baeckea frutescens. Antibacterial food application plant extract phytochemicals stability.
Antimicrobial activity of ethanol leaf extracts of. European journal of botany plant sciences and phytology vol. Review plant extracts for the control of bacterial growth. Single component of antibacterial plant extract has high purity and obvious effect to be widely used in medcine and cleansers. Antibacterial activity of various plants extracts against. Antibacterial activity of some plant extracts used in folk medicine. This activity supports their use in treatment of infections caused by such resistant bacteria. Concerns regarding the safety and toxicity of medicinal plants an overview abstract all over the world, especially in developing countries herbal drugs are playing an available. The resulting inhibition zones were measured in millimeters mm. Bacterial inoculums were prepared by the genus that falls under this enormous family.
Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria article pdf available in brazilian journal of microbiology 314 october 2000 with 1,742 reads. This study was based on screening antibacterial activity of the ethanol extract of baeckea frutescens l. Human resource development department, council of scienti. Samples of plant extracts or pure compounds can be added to fungal spores collected from solid cultures, placed on glass slides, and incubated at an. Evaluation of antibacterial effect of some sinai medicinal. Biochemistry graduates to have results from their final. Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and publichealth point of view. Plants have an almost limitless ability to synthesize aromatic substances, most of which are phenols or their oxygensubstituted derivatives. The antibacterial activity was assessed against six. Antibacterial activity of some plant extracts used in folk. The antibacterial susceptibility tests showed that the alkaloid extract had potent antibacterial properties. Pdf antimicrobial activity of plant extracts against.
Preparation of plant extracts from indigenous medicinal plants. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus. Issn phytochemical and antimicrobial evaluation of plant. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. Antimicrobial activity of crude extracts of three medicinal. The antibacterial activity of extracts of nine plant species with good. To this end, new natural antioxidant molecules are being researched in medicinal plants for the role they can play in the prevention of diseases such as cancer. Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants.
In many cases, these substances serve as plant defense. In the present work, selected plants were screened for their potential antibacterial activity. To promote the proper use of herbal medicine and to determine their potential as sources for new drugs, it is essential to study medicinal plants, which have. Antimicrobial activity of plant extracts against bacillus subtilis, staphylococcus aureus and escherichia coli article pdf available in ejournal of chemistry 8s1 december 2011 with 844 reads. Antimicrobial activity of thespesia populnea soland. Pdf antibacterial activity of medicinal plant extracts. In many cases, these substances serve as plant defense mechanisms.
Four different solvents were used for the extraction. Natural products, such as plant extract, either as pure compounds or as standardized extracts, provide unlimited opportunities for con. Delonix elata, enicostemma axillare, merremia tridentata, mollugo cerviana and solanum incanum used in. Antibacterial and antifungal activities of thespesia populnea. The antibacterial activity was observed by measuring the.
Antibacterial and antifungal activities of thespesia. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Antibacterial plant extract manufacturers and supplier. Antibacterial activity of aqueous extract of calotropis. The antibacterial plant extract use natural herb as raw materials. In recent times, persistent claimed of efficacy of the crude extracts of a. There is a general belief amongst the consumers globally that herbal drugs are always safe because they are natural.
Efficacy of natural plant extracts 63 hop humulus lupulus, 15% licorice root glycyr rhiza uralensis, 15% curcuma curcuma xanthor rhiza, 10% clove bud syzygium aromaticum, 5% oregano leaf, and 5% ajowain seed trachysper mum ammi, obtained from flavex germany. Compared to the rest of the extracts, it also displayed a superior zone of inhibition against the aforementioned bacteria. Antibacterial activity of some flavonoids and organic acids. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Plant based natural products traditionally known to combat microbial infections are expected to play a big role in this regard 2005. Our ability to effectively treat disease is dependent on the development of new pharmaceuticals, and one potential source of novel drugs is traditional medicine. Medicinal plants as source of antibacterial agents to. The antibacterial activity of selected plants towards. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts journal of microbiological methods. Antimicrobial activity of fermented theobroma cacao pod. Antibacterial activities of plant extract this was done using the agar well diffusion method as described by olutiola et al. Antimicrobial activity of crude extracts of some plant leaves.
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